Purification and Characterization of a Secreted Purple Phosphatase from Soybean Suspension Cultures
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چکیده
منابع مشابه
Isolation and characterization of organelles from soybean suspension cultures.
Whole homogenates from cells of Glycine max grown in suspension culture were centrifuged on linear sucrose gradients. Assays for marker enzymes showed that distinct peaks enriched in particular organelles were separated as follows: endoplasmic reticulum (density 1.10 g/cm(3), NADH-cytochrome-c reductase), Golgi membranes (density 1.12 g/cm(3), inosine diphosphatase), mitochondria (density 1.18-...
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The major acid phosphatase [EC 3.1.3.2J isozyme secreted by maize endosperm cultures was purified from concentrated culture filtrates by (NH4)2S04 fractionation and chromatography on columns of diethylaminolethylSephadex A-25, concanavalin A-Sepharose, and Sephacryl S200. The native and subunit M, value's for the purified phosphatase were 29000 and 31500, respectively. The purified enzyme conta...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1992
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.99.2.391